Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Dmitriev, Ruslan I. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Cham : Springer International Publishing : Imprint: Springer, 2017.
Edición:1st ed. 2017.
Colección:Advances in Experimental Medicine and Biology, 1035
Materias:
Acceso en línea:https://doi.org/10.1007/978-3-319-67358-5
Tabla de Contenidos:
  • Current state-of-the-art 3D tissue models and their compatibility with live cell imaging
  • Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation
  • Quantitative live cell FLIM imaging in three dimensions
  • Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview
  • Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues
  • Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture
  • Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2
  • Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell
  • Quantitative imaging of Ca2+ by 3D-FLIM in live tissues
  • Live cell imaging of viscosity in 3D tumour cell models
  • Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy
  • Raman imaging microscopy for quantitative analysis of biological samples.