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05115nam a22003135i 4500 |
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978-3-642-57206-7 |
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20191028112231.0 |
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cr nn 008mamaa |
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121227s2000 gw | s |||| 0|eng d |
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|a 9783642572067
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|a 10.1007/978-3-642-57206-7
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Nonradioactive Analysis of Biomolecules
|c edited by Christoph Kessler.
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| 250 |
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|a 2nd ed. 2000.
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| 260 |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 2000.
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| 300 |
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|a XVIII, 750 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Springer Lab Manuals
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|a 1 General Aspects of Nonradioactive Analysis of Biomolecules -- 2 Overview of Nonradioactive Labeling Systems -- 3 Overview on the Digoxigenin:Anti-Digoxigenin (DIG) System -- 4 Labeling and Detection of Nucleic Acids -- 5 Labeling and Detection of Proteins and Glycoproteins -- 6 Labeling and Detection of Nucleic Acids -- 7 Labeling of Proteins DuringIn VitroTranslation -- 8 Labeling and Detection of Proteins and Glycoproteins -- 9 In Vitro Labeling of DNA Probes with 5-BrdU -- 10 AAF-labeling of DNA and Oligonucleotides -- 11 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Conjugates -- 12 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection -- 13 The SNAP System -- 14 Overview on Nonradioactive Detections Systems -- 15 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems -- 16 Indigo / Tetrazolium Dyes -- 17 Azo Dyes -- 18 Chemiluminescent Detection with Horseradish Peroxidase and Luminol -- 19 Chemiluminescence: Properties of 1,2-Dioxetane Chemiluminescence -- 20 Electrochemiluminescence: Ruthenium Complexes -- 21 Bioluminescence: D-Luciferin as Substrate -- 22 Labeling of Biomolecules with Fluorophores -- 23 Time-Resolved Fluorescence -- 24 Overview of Amplification Systems -- 25 Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Basic Principles -- 26 PCR: Overview on Application Formats in Research and Clinical Diagnosis -- 27 PCR Amplification for the Generation of DIG-Labeled Probes -- 28 In Situ PCR Amplification of cDNA -- 29 SDA Target Amplification -- 30 In Situ Strand Displacement Amplification -- 31 Isothermal Amplification of RNA by Transcription-Mediated Amplification (TMA) -- 32 Ligase Chain Reaction -- 33 Branched DNA (bDNA) Technology -- 34 rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification -- 35 PRINS: Primed In Situ Labeling and Hybridization in one Step -- 36 Overview on Amplification Formats -- 37 Overview on Factors Influencing Nucleic Acid Hybridization -- 38 Dot, Southern, and Northern Blots -- 39 PNA Pre-Gel Hybridization - An Alternative to Southern Blotting -- 40 Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements -- 41 Detection of Proteins and Glycoproteins on Western Blots -- 42 PNA as Specific Probe for In Situ Hybridization to Metaphase Chromosomes -- 43 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays -- 44 Non-Fluorescent Differentiation of Viral and Chromosomal Nucleic Acids in Individual Nuclei -- 45 Virus Detection in Biopsy Specimens -- 46 Mapping of Polytene Chromosomes -- 47 Fluorescence In Situ Hybridization on Banded Chromosomes -- 48 Spectral Karyotyping: New Tools for Multicolor Chromosome Analysis -- 49 Chromosome Analysis by Multiplex-FISH (M-FISH) -- 50 Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos -- 51 Double Labeling of mRNA and Proteins in Drosophila Embryos -- 52 Detection of DNA/RNA Target/Probe Complexes with DNA/RNA-Specific Antibodies -- 53 Molecular Beacons: Hybridization Probes for Detection of Nucleic Acids in Homogeneous Solutions -- 54 Detection of DIG-Labeled Amplicons on Streptavidin-Coated Microtiter Plates -- 55 A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella -- 56 PCR-Coupled Activation of Intercalating Dyes -- 57 Simultaneous SDA and Fluorescence Polarization Detection -- 58 Large-Scale Genomic Sequencing Using Four Color Fluorescent Detection - Principles and Protocols -- 59 DIG DNA Sequencing with Chemiluminescent or Dye Substrates -- 60 DNA Sequencing: Chemiluminescent Detection with the 1,2 Dioxane CSPD -- 61 Direct-Blotting-Electrophoresis (DBE) for DNA Sequencing -- 62 Sequence Analysis of Nucleic Acids by Mass Spectrometry -- 63 Mismatch Analysis by PNA Arrays -- 64 Oligonucleotide Arrays for the Detection of ras Mutations -- 65 Use of Oligonucleotide Arrays for Gene Expression Monitoring.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Biochemistry.
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| 650 |
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4 |
|a Cell Biology.
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| 650 |
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4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Kessler, Christoph.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-3-642-57206-7
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