Nonradioactive Analysis of Biomolecules
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2000.
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| Edición: | 2nd ed. 2000. |
| Colección: | Springer Lab Manuals
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-57206-7 |
Tabla de Contenidos:
- 1 General Aspects of Nonradioactive Analysis of Biomolecules
- 2 Overview of Nonradioactive Labeling Systems
- 3 Overview on the Digoxigenin:Anti-Digoxigenin (DIG) System
- 4 Labeling and Detection of Nucleic Acids
- 5 Labeling and Detection of Proteins and Glycoproteins
- 6 Labeling and Detection of Nucleic Acids
- 7 Labeling of Proteins DuringIn VitroTranslation
- 8 Labeling and Detection of Proteins and Glycoproteins
- 9 In Vitro Labeling of DNA Probes with 5-BrdU
- 10 AAF-labeling of DNA and Oligonucleotides
- 11 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Conjugates
- 12 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection
- 13 The SNAP System
- 14 Overview on Nonradioactive Detections Systems
- 15 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems
- 16 Indigo / Tetrazolium Dyes
- 17 Azo Dyes
- 18 Chemiluminescent Detection with Horseradish Peroxidase and Luminol
- 19 Chemiluminescence: Properties of 1,2-Dioxetane Chemiluminescence
- 20 Electrochemiluminescence: Ruthenium Complexes
- 21 Bioluminescence: D-Luciferin as Substrate
- 22 Labeling of Biomolecules with Fluorophores
- 23 Time-Resolved Fluorescence
- 24 Overview of Amplification Systems
- 25 Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Basic Principles
- 26 PCR: Overview on Application Formats in Research and Clinical Diagnosis
- 27 PCR Amplification for the Generation of DIG-Labeled Probes
- 28 In Situ PCR Amplification of cDNA
- 29 SDA Target Amplification
- 30 In Situ Strand Displacement Amplification
- 31 Isothermal Amplification of RNA by Transcription-Mediated Amplification (TMA)
- 32 Ligase Chain Reaction
- 33 Branched DNA (bDNA) Technology
- 34 rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification
- 35 PRINS: Primed In Situ Labeling and Hybridization in one Step
- 36 Overview on Amplification Formats
- 37 Overview on Factors Influencing Nucleic Acid Hybridization
- 38 Dot, Southern, and Northern Blots
- 39 PNA Pre-Gel Hybridization - An Alternative to Southern Blotting
- 40 Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements
- 41 Detection of Proteins and Glycoproteins on Western Blots
- 42 PNA as Specific Probe for In Situ Hybridization to Metaphase Chromosomes
- 43 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays
- 44 Non-Fluorescent Differentiation of Viral and Chromosomal Nucleic Acids in Individual Nuclei
- 45 Virus Detection in Biopsy Specimens
- 46 Mapping of Polytene Chromosomes
- 47 Fluorescence In Situ Hybridization on Banded Chromosomes
- 48 Spectral Karyotyping: New Tools for Multicolor Chromosome Analysis
- 49 Chromosome Analysis by Multiplex-FISH (M-FISH)
- 50 Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos
- 51 Double Labeling of mRNA and Proteins in Drosophila Embryos
- 52 Detection of DNA/RNA Target/Probe Complexes with DNA/RNA-Specific Antibodies
- 53 Molecular Beacons: Hybridization Probes for Detection of Nucleic Acids in Homogeneous Solutions
- 54 Detection of DIG-Labeled Amplicons on Streptavidin-Coated Microtiter Plates
- 55 A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella
- 56 PCR-Coupled Activation of Intercalating Dyes
- 57 Simultaneous SDA and Fluorescence Polarization Detection
- 58 Large-Scale Genomic Sequencing Using Four Color Fluorescent Detection - Principles and Protocols
- 59 DIG DNA Sequencing with Chemiluminescent or Dye Substrates
- 60 DNA Sequencing: Chemiluminescent Detection with the 1,2 Dioxane CSPD
- 61 Direct-Blotting-Electrophoresis (DBE) for DNA Sequencing
- 62 Sequence Analysis of Nucleic Acids by Mass Spectrometry
- 63 Mismatch Analysis by PNA Arrays
- 64 Oligonucleotide Arrays for the Detection of ras Mutations
- 65 Use of Oligonucleotide Arrays for Gene Expression Monitoring.