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03163nam a22003495i 4500 |
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978-3-642-71543-3 |
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20191027043032.0 |
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cr nn 008mamaa |
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121227s1986 gw | s |||| 0|eng d |
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|a 9783642715433
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| 024 |
7 |
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|a 10.1007/978-3-642-71543-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Membrane Proteins
|b Isolation and Characterization /
|c edited by Angelo Azzi, Lanfranco Masotti, Arnaldo Vecli.
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| 246 |
3 |
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|a With contributions by numerous experts
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| 250 |
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|a 1st ed. 1986.
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| 260 |
# |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1986.
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| 300 |
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|a XII, 182 p. 3 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|a I. Membrane Proteins: Function, Purification, and Characterization -- Purification of Cytochrome c Reductase and Oxidase by Affinity Chromatography -- Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient Centrifugation -- The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranes -- Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporation into Lipids -- Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphatase from Rhodospirillum rubrum -- The Function of Transmembrane Channels: Ion Transport Studies by 23Na NMR -- Membrane Signal Transduction via Protein Kinase C -- Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochondrial Membrane -- II. Lipid-Protein Interaction -- The Effect of Polypeptides on the Fluidity of Lipid Bilayers -- Polypeptide-Lipid Interactions as Studied by 13C NMR -- Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipids -- III. Protein Modification -- Two Examples of Selective Fluorescent Labeling of SH-Groups with Eosin-5-Maleimide: The ADP/ATP Translocator and the Cytochrome c Oxidase Subunit III of Bovine Heart Mitochondria -- Hydrophobic Photolabeling with 125I-TID of Red Blood Cell Membranes -- Use of Fluorescent Probes of the Adenine Nucleotide Carrier for Binding Studies and Analysis of Conformational Changes -- Spin Labeling of Membranes and Membrane Proteins -- IV. Protein Reconstitution -- Functional Reconstitution of the Mitochondrial Cytochrome b-c1 Complex: Effect of Cholesterol -- Changes of the Membrane Surface Potential Measured by Amphiphilic Fluorescent and ESR Probes -- Reconstitution of Cytochrome c Oxidase -- Measurement of Pyruvate Transport in Mitochondria.
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| 650 |
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|a Biochemistry.
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| 650 |
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0 |
|a Biophysics.
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| 650 |
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|a Biological physics.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Biological and Medical Physics, Biophysics.
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| 700 |
1 |
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|a Azzi, Angelo.
|e editor.
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| 700 |
1 |
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|a Masotti, Lanfranco.
|e editor.
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| 700 |
1 |
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|a Vecli, Arnaldo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-3-642-71543-3
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