Membrane Proteins Isolation and Characterization /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1986.
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| Edición: | 1st ed. 1986. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-71543-3 |
Tabla de Contenidos:
- I. Membrane Proteins: Function, Purification, and Characterization
- Purification of Cytochrome c Reductase and Oxidase by Affinity Chromatography
- Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient Centrifugation
- The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranes
- Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporation into Lipids
- Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphatase from Rhodospirillum rubrum
- The Function of Transmembrane Channels: Ion Transport Studies by 23Na NMR
- Membrane Signal Transduction via Protein Kinase C
- Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochondrial Membrane
- II. Lipid-Protein Interaction
- The Effect of Polypeptides on the Fluidity of Lipid Bilayers
- Polypeptide-Lipid Interactions as Studied by 13C NMR
- Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipids
- III. Protein Modification
- Two Examples of Selective Fluorescent Labeling of SH-Groups with Eosin-5-Maleimide: The ADP/ATP Translocator and the Cytochrome c Oxidase Subunit III of Bovine Heart Mitochondria
- Hydrophobic Photolabeling with 125I-TID of Red Blood Cell Membranes
- Use of Fluorescent Probes of the Adenine Nucleotide Carrier for Binding Studies and Analysis of Conformational Changes
- Spin Labeling of Membranes and Membrane Proteins
- IV. Protein Reconstitution
- Functional Reconstitution of the Mitochondrial Cytochrome b-c1 Complex: Effect of Cholesterol
- Changes of the Membrane Surface Potential Measured by Amphiphilic Fluorescent and ESR Probes
- Reconstitution of Cytochrome c Oxidase
- Measurement of Pyruvate Transport in Mitochondria.