Nucleic Acids in the Environment
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1995.
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| Edición: | 1st ed. 1995. |
| Colección: | Springer Lab Manuals
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-79050-8 |
Tabla de Contenidos:
- 1 Introduction to Nucleic Acids in the Environment: Methods and Applications
- 1.1 Introduction
- 1.2 Cell Lysis, Nucleic Acid Extraction and Purification Protocols
- 1.3 Analysis of Environmental Nucleic Acids
- 1.4 Concluding Remarks
- References
- 2 Recovery of Bacterial Cells from Soil
- 2.1 Introduction
- 2.2 Strategies for Cell Extraction
- 2.3 Recommended Methods
- 2.4 Practical Experiences and Recommendations
- References
- 3 Extraction, Purification, and Analysis of DNA from Soil Bacteria
- 3.1 Introduction
- 3.2 Bacterial DNA
- 3.3 Direct Extraction of DNA from Soil
- 3.4 Purity Control and Characterization of Soil Bacterial DNA
- References
- 4 Extraction and Analysis of Microbial DNA from Soil
- 4.1 Introduction
- 4.2 DNA Extraction Methods
- 4.3 Evaluation of DNA Extraction Methods
- 4.4 Methods for Analysis of DNA from Soil
- 4.5 Concluding Remarks
- References
- 5 Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants
- 5.1 Introduction
- 5.2 Sample Preparation
- 5.3 Extraction, Purification and Quantification of DNA from the Rhizosphere
- 5.4 PCR Analysis of Bacterial DNA from the Rhizosphere and Rhizoplane of Plants
- 5.5 Concluding Remarks
- References
- 6 Extraction of DNA from the Phyllosphere
- 6.1 Introduction
- 6.2 Materials, Methods and Protocols
- 6.3 Concluding Remarks
- References
- 7 Specific DNA Sequences for Detection of Soil Bacteria
- 7.1 Introduction
- 7.2 Objectives and Limitations
- 7.3 Target DNA, DNA Extraction and Detection in Soil
- 7.4 Detailed Procedures
- 7.5 Application of Methodology
- References
- 8 PCR Amplification of DNA from Root Nodules
- 8.1 Introduction
- 8.2 Methods for Detection of Rhizobia
- 8.3 Detection of Root Nodule Bacteria by PCR
- 8.4 Conclusions
- References
- 9 Extraction of DNA and RNA from Aquatic Environments
- 9.1 Introduction
- 9.2 DNA as a Biomass and Ecological Indicator
- 9.3 RNA as a Biomass and Ecological Indicator
- 9.4 Biomass and Activity Measurements of Aquatic Microorganisms using RNA and RNA /DNA Ratios
- 9.5 Extraction of Nucleic Acids for Molecular Biological Studies
- 9.6 Molecular Methods for Studying RNA in Aquatic Environments
- 9.7 Extraction of DNA from Aquatic Microbial Populations
- 9.8 Extraction of RNA from Aquatic Microbial Populations
- References
- 10 PCR Amplification of DNA Recovered from the Aquatic Environment
- 10.1 Introduction
- 10.2 Concentration of Water Samples from Aquatic Environments
- 10.3 Sample Preparation for PCR Amplification
- 10.4 Processing of Dissolved DNA in the Aquatic Environment
- 10.5 Processing of Nucleic Acids from Biofilms
- 10.6 PCR Amplification
- 10.7 Identification and Analysis of the Amplified DNA
- 10.8 PCR Contamination Control
- 10.9 Enhancing the Specificity of the PCR Reaction
- 10.10 Use of Arbitrarily Primed PCR (Ap-PCR) in Environmental Microbiology
- 10.11 In situ PCR Amplification
- 10.12 The Use of PCR in Aquatic Environments
- 10.13 Detection of Viruses in Water
- 10.14 Discussion
- References
- 11 Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity
- 11.1 Introduction
- 11.2 DNA Extraction
- 11.3 Amplification of 16S Ribosomal RNA Gene Sequences
- 11.4 Cloning and Sequencing
- 11.5 Sequences Obtained from Marine Sediments and Coastal Waters
- References
- 12 Application of the PCR for Detection of Antibiotic Resistance Genes in Environmental Samples
- 12.1 Introduction
- 12.2 Selection of PCR Primers and Gene Probes for Specific Detection of sat and nptII Genes
- 12.3 Detection of Antibiotic Resistance Genes in Bacterial Isolates Using PCR
- 12.4 Detection of Antibiotic Resistance Genes Using DNA Extraction and PCR
- 12.5 Detection of Antibiotic Resistance Genes in Total Community DNA Extracts from Environmental Habitats.
- 12.6 Conclusions
- References.