Nucleic Acids in the Environment

Nucleic Acids in the Environment

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Trevors, Jack T. (Editor), Elsas, J.Dick van. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1995.
Edition:1st ed. 1995.
Series:Springer Lab Manuals
Subjects:
Geography.
Geoecology.
Environmental geology.
Nature conservation.
Geography, general.
Geoecology/Natural Processes.
Nature Conservation.
Online Access:https://doi.org/10.1007/978-3-642-79050-8
Table of Contents:
  • 1 Introduction to Nucleic Acids in the Environment: Methods and Applications
  • 1.1 Introduction
  • 1.2 Cell Lysis, Nucleic Acid Extraction and Purification Protocols
  • 1.3 Analysis of Environmental Nucleic Acids
  • 1.4 Concluding Remarks
  • References
  • 2 Recovery of Bacterial Cells from Soil
  • 2.1 Introduction
  • 2.2 Strategies for Cell Extraction
  • 2.3 Recommended Methods
  • 2.4 Practical Experiences and Recommendations
  • References
  • 3 Extraction, Purification, and Analysis of DNA from Soil Bacteria
  • 3.1 Introduction
  • 3.2 Bacterial DNA
  • 3.3 Direct Extraction of DNA from Soil
  • 3.4 Purity Control and Characterization of Soil Bacterial DNA
  • References
  • 4 Extraction and Analysis of Microbial DNA from Soil
  • 4.1 Introduction
  • 4.2 DNA Extraction Methods
  • 4.3 Evaluation of DNA Extraction Methods
  • 4.4 Methods for Analysis of DNA from Soil
  • 4.5 Concluding Remarks
  • References
  • 5 Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants
  • 5.1 Introduction
  • 5.2 Sample Preparation
  • 5.3 Extraction, Purification and Quantification of DNA from the Rhizosphere
  • 5.4 PCR Analysis of Bacterial DNA from the Rhizosphere and Rhizoplane of Plants
  • 5.5 Concluding Remarks
  • References
  • 6 Extraction of DNA from the Phyllosphere
  • 6.1 Introduction
  • 6.2 Materials, Methods and Protocols
  • 6.3 Concluding Remarks
  • References
  • 7 Specific DNA Sequences for Detection of Soil Bacteria
  • 7.1 Introduction
  • 7.2 Objectives and Limitations
  • 7.3 Target DNA, DNA Extraction and Detection in Soil
  • 7.4 Detailed Procedures
  • 7.5 Application of Methodology
  • References
  • 8 PCR Amplification of DNA from Root Nodules
  • 8.1 Introduction
  • 8.2 Methods for Detection of Rhizobia
  • 8.3 Detection of Root Nodule Bacteria by PCR
  • 8.4 Conclusions
  • References
  • 9 Extraction of DNA and RNA from Aquatic Environments
  • 9.1 Introduction
  • 9.2 DNA as a Biomass and Ecological Indicator
  • 9.3 RNA as a Biomass and Ecological Indicator
  • 9.4 Biomass and Activity Measurements of Aquatic Microorganisms using RNA and RNA /DNA Ratios
  • 9.5 Extraction of Nucleic Acids for Molecular Biological Studies
  • 9.6 Molecular Methods for Studying RNA in Aquatic Environments
  • 9.7 Extraction of DNA from Aquatic Microbial Populations
  • 9.8 Extraction of RNA from Aquatic Microbial Populations
  • References
  • 10 PCR Amplification of DNA Recovered from the Aquatic Environment
  • 10.1 Introduction
  • 10.2 Concentration of Water Samples from Aquatic Environments
  • 10.3 Sample Preparation for PCR Amplification
  • 10.4 Processing of Dissolved DNA in the Aquatic Environment
  • 10.5 Processing of Nucleic Acids from Biofilms
  • 10.6 PCR Amplification
  • 10.7 Identification and Analysis of the Amplified DNA
  • 10.8 PCR Contamination Control
  • 10.9 Enhancing the Specificity of the PCR Reaction
  • 10.10 Use of Arbitrarily Primed PCR (Ap-PCR) in Environmental Microbiology
  • 10.11 In situ PCR Amplification
  • 10.12 The Use of PCR in Aquatic Environments
  • 10.13 Detection of Viruses in Water
  • 10.14 Discussion
  • References
  • 11 Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity
  • 11.1 Introduction
  • 11.2 DNA Extraction
  • 11.3 Amplification of 16S Ribosomal RNA Gene Sequences
  • 11.4 Cloning and Sequencing
  • 11.5 Sequences Obtained from Marine Sediments and Coastal Waters
  • References
  • 12 Application of the PCR for Detection of Antibiotic Resistance Genes in Environmental Samples
  • 12.1 Introduction
  • 12.2 Selection of PCR Primers and Gene Probes for Specific Detection of sat and nptII Genes
  • 12.3 Detection of Antibiotic Resistance Genes in Bacterial Isolates Using PCR
  • 12.4 Detection of Antibiotic Resistance Genes Using DNA Extraction and PCR
  • 12.5 Detection of Antibiotic Resistance Genes in Total Community DNA Extracts from Environmental Habitats.
  • 12.6 Conclusions
  • References.

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