Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods /
| Corporate Author: | |
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| Other Authors: | , , |
| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Springer Protocols Handbooks,
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| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-3-662-49131-7 |
Table of Contents:
- Introduction: Single cell biotechnology for uncultured microorganisms
- Single-cell cultivation in microfluidic devices
- Single-cell bacterial bioreporter assays to measure hydrocarbons
- Whole genome amplification of single bacterial cells
- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria
- Localisation of proteins within intact bacterial cells using fluorescent protein fusions
- Raman-FISH
- Single-cell metabolomics
- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division
- Single cell biotechnology for uncultured microorganisms
- Single cell and systems biology tools for biofuel production
- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT)
- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity
- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence. .