Hydrocarbon and Lipid Microbiology Protocols Genetic, Genomic and System Analyses of Communities /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: McGenity, Terry J. (Editor), Timmis, Kenneth N. (Editor), Nogales, Balbina. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2017.
Edition:1st ed. 2017.
Series:Springer Protocols Handbooks,
Subjects:
Online Access:https://doi.org/10.1007/978-3-662-50450-5
Table of Contents:
  • Introduction to genetic, genomic and system analyses for communities
  • Genomic analysis of pure cultures and communities
  • Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems
  • Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems
  • Prokaryotic metatranscriptomics
  • Defining a pipeline for metaproteomic analyses
  • Generating enriched metagenomes from active microorganisms with DNA stable isotope probing
  • DNA and RNA-based stable isotope probing of hydrocarbon degraders
  • Protein stable isotope probing (Protein-SIP)
  • Protein extraction from contaminated soils and sediments
  • Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities
  • Physiological modelling of complex bacterial communities
  • Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants.