Plant Tissue Culture Manual Supplement 6 /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
1996.
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| Edición: | 1st ed. 1996. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-94-009-0181-0 |
Tabla de Contenidos:
- Section A: Basic Techniques — Cell & Tissue Culture of Model Species
- 1. Media preparation
- 2. The initiation and maintenance of callus cultures of carrot & tobacco
- 3. The initiation and maintenance of plant cell suspension cultures
- 4. Shoot cultures and root cultures of tobacco
- 5. Somatic embryogenesis in orchardgrass
- 6. Arabidopsis regeneration and transformation (leaf & cotyledon expiant system)
- 7. Arabidopsis protoplast transformation and regeneration
- 8. Arabidopsis regeneration and transformation (root expiant system)
- 9. Somatic embryogenesis in carrot
- 10. Low density cultures: microdroplets and single-cell nurse cultures
- 11. Tobacco protoplast isolation, culture and regeneration
- Section B: Tissue Culture & Transformation of Crop Species
- 1. Embryogenic callus, cell suspension and protoplast cultures of cereals
- 2. Transformation and regeneration of rice protoplasts
- 3. Transformation and regeneration of orchardgrass protoplasts
- 4. Transformation and regeneration of oilseed rape protoplasts
- 5. Regeneration and transformation of potato by Agrobacterium tumefaciens
- 6. Transformation of tomato with Agrobacterium tumefaciens
- 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens
- 8. Regeneration and transformation of apple (Malus pumila Mill.)
- 9. Transformation and regeneration of maize protoplasts
- 10. Regeneration and transformation of barley protoplasts
- 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation
- 12. Production of fertile transgenic wheat by microprojectile bombardment
- 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment
- Section C: Propagation & Conservation of Germplasm
- 1. Clonal propagation of orchids
- 2. Clonal propagation of palms
- 3. Clonal propagation of conifers
- 4. Cytological techniques
- 5. Restriction fragment analysis of somaclones
- 6. Virus elimination and testing
- 7. Clonal propagation of Citrus
- 8. Clonal propagation of eucalypts
- 9. Cryopreservation of plant tissue cultures: the example of embryogénie tissue cultures from conifers
- Section D: Direct Gene Transfer & Protoplast Fusion
- 1. Gene transfer by particle bombardment
- 2. Transformation of pollen by particle bombardment
- 3. Electrical fusion of protoplasts
- 4. Cybrid production and selection
- 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids
- 6. RFLP analysis of organellar genomes in somatic hybrids
- 7. Isolation and uptake of plant nucle
- 8. In situ hybridization to plant metaphase chromosomes: Radioactive and non-radioactive detection of repetitive and low copy number genes
- 9. Chemical fusion of protoplasts
- Section E: Reproductive Tissues
- 1. In vitro fertilisation of maize
- 2. Endosperm culture
- 3. Endosperm culture
- 4. Hybrid embryo rescue
- 5. In vitro culture of Brassica juncea zygotic proembryos
- Section F: Mutant Selection
- 1. Use of chemical and physical mutagens in vitro
- 2. In vitro culture, mutant selection, genetic analysis and transformation of Physcomitrella patens
- Section G: Secondary Metabolites
- 1. Tropane alkaloid biosynthesis in vitro
- 2. Anthocyanin biosynthesis in vitro
- 3. Biosynthesis of monoterpene indole alkaloids in vitro
- Section H: Tissue Culture Techniques for Fundamental Studies
- 1. Establishment of photoautotrophic cell cultures
- 2. Zinnia mesophyll culture system to study xylogenesis
- 3. Cell cycle studies: induction of synchrony in suspension cultures of Catharanthus roseus cells H. Kodama
- 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns
- 5. In vitro infection of Arabidopsis with nematodes.