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04163nam a22002895i 4500 |
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978-94-011-5750-6 |
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20191025162046.0 |
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121227s1997 ne | s |||| 0|eng d |
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|a 9789401157506
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|a 10.1007/978-94-011-5750-6
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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100 |
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|a Pierik, R.L.M.
|e author.
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|a In Vitro Culture of Higher Plants
|c by R.L.M Pierik.
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250 |
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|a 1st ed. 1997.
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|a Dordrecht :
|b Springer Netherlands :
|b Imprint: Springer,
|c 1997.
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|a V, 348 p.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a 2. Introduction -- 2.1. Outline -- 2.2. Abbreviations and glossary -- 3. History -- 3.1. Introduction -- 3.2. Developments in the Netherlands and other countries -- 4. Types of cultures -- 5. Laboratory equipment -- 5.1. Supplies -- 5.2. The laminar air-flow cabinet -- 5.3. Sterilization of nutrient media -- 5.4. Preparation room -- 5.5. Cleaning glassware -- 5.6. Robots in the plant tissue culture laboratory -- 6. Preparation and composition of nutrient media -- 6.1. Introduction -- 6.2. Glassware and plastics -- 6.3. Preparation -- 6.4. Composition -- 6.5. Commercially prepared media -- 6.6. Storage of nutrient media -- 7. Closure of test tubes and flasks -- 8. Care of plant material -- 9. Sterilization of plant material -- 9.1. Introduction -- 9.2. Chemical sterilization -- 9.3. Apparently sterile cultures -- 9.4. Internal infections -- 9.5. Symbiotic cultures -- 10. Isolation, inoculation and subculturing -- 10.1. Introduction -- 10.2. Isolation -- 10.3. Inoculation -- 10.4. Subculturing -- 11. Mechanization -- 12. The influence of plant material on growth and development -- 13. The influence of physical factors on growth and development -- 13.1. The culture room -- 13.2. Discussion of special physical factors -- 14. The transfer from nutrient medium to soil -- 15. Aids to study -- 15.1. Literature study -- 15.2. Societies and associations -- 15.3. Laboratory notebook, photographs and slides -- 16. Embryo culture -- 16.1. Introduction -- 16.2. Techniques -- 16.3. Factors affecting the success of embryo culture -- 16.4. Practical applications -- 17. Germination of orchid seeds -- 17.1. Introduction -- 17.2. Factors affecting germination and growth -- 18. Vegetative propagation of orchids -- 18.1. Introduction -- 18.2. Meristem culture -- 18.3. Other methods of propagation -- 18.4. Variation arising during culture -- 18.5. Practical applications -- 19. Production of disease-free plants -- 19.1. Introduction -- 19.2. Production of virus-free plants -- 19.3. Production of bacteria- and fungal-free plants by meristem culture -- 20. Vegetative propagation -- 20.1. General introduction -- 20.2. Single-node culture -- 20.3. The axillary bud method -- 20.4. Regeneration of explants -- 20.5. Callus induction, callus culture and regeneration of organs and embryos -- 20.6. Regeneration of plants from single cells -- 20.7. Synthetic seeds -- 21. Somaclonal variation -- 22. Test tube fertilization -- 23. Production of haploids -- 23.1. General introduction -- 23.2. Obtaining haploids in vitro -- 23.3. Problems associated with haploid induction -- 24. Genetic manipulation -- 24.1. General introduction -- 24.2. Description -- 24.3. Prerequisites for the use of genetic manipulation -- 24.4. Somatic hybridization -- 24.5. The relevance of somatic hybridization -- 24.6. Disadvantages and problems of somatic hybridization -- 24.7. Selection procedures after somatic hybridization -- 24.8. Selection of mutants -- 24.9. Transformation by Agrobacterium tumefaciens -- 25. Miscellaneous applications -- 25.1. In phytopathology -- 25.2. In plant breeding -- 25.3. The biosynthesis of substances in vitro -- 26. In vitro cloning of plants in the Netherlands -- 27. Index -- 28. Literature cited.
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650 |
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|a Plant science.
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650 |
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|a Botany.
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650 |
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|a Plant Sciences.
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710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1007/978-94-011-5750-6
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