In Vitro Culture of Higher Plants

In Vitro Culture of Higher Plants

Bibliographic Details
Main Author: Pierik, R.L.M. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Dordrecht : Springer Netherlands : Imprint: Springer, 1997.
Edition:1st ed. 1997.
Subjects:
Plant science.
Botany.
Plant Sciences.
Online Access:https://doi.org/10.1007/978-94-011-5750-6
Table of Contents:
  • 2. Introduction
  • 2.1. Outline
  • 2.2. Abbreviations and glossary
  • 3. History
  • 3.1. Introduction
  • 3.2. Developments in the Netherlands and other countries
  • 4. Types of cultures
  • 5. Laboratory equipment
  • 5.1. Supplies
  • 5.2. The laminar air-flow cabinet
  • 5.3. Sterilization of nutrient media
  • 5.4. Preparation room
  • 5.5. Cleaning glassware
  • 5.6. Robots in the plant tissue culture laboratory
  • 6. Preparation and composition of nutrient media
  • 6.1. Introduction
  • 6.2. Glassware and plastics
  • 6.3. Preparation
  • 6.4. Composition
  • 6.5. Commercially prepared media
  • 6.6. Storage of nutrient media
  • 7. Closure of test tubes and flasks
  • 8. Care of plant material
  • 9. Sterilization of plant material
  • 9.1. Introduction
  • 9.2. Chemical sterilization
  • 9.3. Apparently sterile cultures
  • 9.4. Internal infections
  • 9.5. Symbiotic cultures
  • 10. Isolation, inoculation and subculturing
  • 10.1. Introduction
  • 10.2. Isolation
  • 10.3. Inoculation
  • 10.4. Subculturing
  • 11. Mechanization
  • 12. The influence of plant material on growth and development
  • 13. The influence of physical factors on growth and development
  • 13.1. The culture room
  • 13.2. Discussion of special physical factors
  • 14. The transfer from nutrient medium to soil
  • 15. Aids to study
  • 15.1. Literature study
  • 15.2. Societies and associations
  • 15.3. Laboratory notebook, photographs and slides
  • 16. Embryo culture
  • 16.1. Introduction
  • 16.2. Techniques
  • 16.3. Factors affecting the success of embryo culture
  • 16.4. Practical applications
  • 17. Germination of orchid seeds
  • 17.1. Introduction
  • 17.2. Factors affecting germination and growth
  • 18. Vegetative propagation of orchids
  • 18.1. Introduction
  • 18.2. Meristem culture
  • 18.3. Other methods of propagation
  • 18.4. Variation arising during culture
  • 18.5. Practical applications
  • 19. Production of disease-free plants
  • 19.1. Introduction
  • 19.2. Production of virus-free plants
  • 19.3. Production of bacteria- and fungal-free plants by meristem culture
  • 20. Vegetative propagation
  • 20.1. General introduction
  • 20.2. Single-node culture
  • 20.3. The axillary bud method
  • 20.4. Regeneration of explants
  • 20.5. Callus induction, callus culture and regeneration of organs and embryos
  • 20.6. Regeneration of plants from single cells
  • 20.7. Synthetic seeds
  • 21. Somaclonal variation
  • 22. Test tube fertilization
  • 23. Production of haploids
  • 23.1. General introduction
  • 23.2. Obtaining haploids in vitro
  • 23.3. Problems associated with haploid induction
  • 24. Genetic manipulation
  • 24.1. General introduction
  • 24.2. Description
  • 24.3. Prerequisites for the use of genetic manipulation
  • 24.4. Somatic hybridization
  • 24.5. The relevance of somatic hybridization
  • 24.6. Disadvantages and problems of somatic hybridization
  • 24.7. Selection procedures after somatic hybridization
  • 24.8. Selection of mutants
  • 24.9. Transformation by Agrobacterium tumefaciens
  • 25. Miscellaneous applications
  • 25.1. In phytopathology
  • 25.2. In plant breeding
  • 25.3. The biosynthesis of substances in vitro
  • 26. In vitro cloning of plants in the Netherlands
  • 27. Index
  • 28. Literature cited.

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