In Vitro Culture of Higher Plants
| Autor principal: | |
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| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
1997.
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| Edición: | 1st ed. 1997. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-94-011-5750-6 |
Tabla de Contenidos:
- 2. Introduction
- 2.1. Outline
- 2.2. Abbreviations and glossary
- 3. History
- 3.1. Introduction
- 3.2. Developments in the Netherlands and other countries
- 4. Types of cultures
- 5. Laboratory equipment
- 5.1. Supplies
- 5.2. The laminar air-flow cabinet
- 5.3. Sterilization of nutrient media
- 5.4. Preparation room
- 5.5. Cleaning glassware
- 5.6. Robots in the plant tissue culture laboratory
- 6. Preparation and composition of nutrient media
- 6.1. Introduction
- 6.2. Glassware and plastics
- 6.3. Preparation
- 6.4. Composition
- 6.5. Commercially prepared media
- 6.6. Storage of nutrient media
- 7. Closure of test tubes and flasks
- 8. Care of plant material
- 9. Sterilization of plant material
- 9.1. Introduction
- 9.2. Chemical sterilization
- 9.3. Apparently sterile cultures
- 9.4. Internal infections
- 9.5. Symbiotic cultures
- 10. Isolation, inoculation and subculturing
- 10.1. Introduction
- 10.2. Isolation
- 10.3. Inoculation
- 10.4. Subculturing
- 11. Mechanization
- 12. The influence of plant material on growth and development
- 13. The influence of physical factors on growth and development
- 13.1. The culture room
- 13.2. Discussion of special physical factors
- 14. The transfer from nutrient medium to soil
- 15. Aids to study
- 15.1. Literature study
- 15.2. Societies and associations
- 15.3. Laboratory notebook, photographs and slides
- 16. Embryo culture
- 16.1. Introduction
- 16.2. Techniques
- 16.3. Factors affecting the success of embryo culture
- 16.4. Practical applications
- 17. Germination of orchid seeds
- 17.1. Introduction
- 17.2. Factors affecting germination and growth
- 18. Vegetative propagation of orchids
- 18.1. Introduction
- 18.2. Meristem culture
- 18.3. Other methods of propagation
- 18.4. Variation arising during culture
- 18.5. Practical applications
- 19. Production of disease-free plants
- 19.1. Introduction
- 19.2. Production of virus-free plants
- 19.3. Production of bacteria- and fungal-free plants by meristem culture
- 20. Vegetative propagation
- 20.1. General introduction
- 20.2. Single-node culture
- 20.3. The axillary bud method
- 20.4. Regeneration of explants
- 20.5. Callus induction, callus culture and regeneration of organs and embryos
- 20.6. Regeneration of plants from single cells
- 20.7. Synthetic seeds
- 21. Somaclonal variation
- 22. Test tube fertilization
- 23. Production of haploids
- 23.1. General introduction
- 23.2. Obtaining haploids in vitro
- 23.3. Problems associated with haploid induction
- 24. Genetic manipulation
- 24.1. General introduction
- 24.2. Description
- 24.3. Prerequisites for the use of genetic manipulation
- 24.4. Somatic hybridization
- 24.5. The relevance of somatic hybridization
- 24.6. Disadvantages and problems of somatic hybridization
- 24.7. Selection procedures after somatic hybridization
- 24.8. Selection of mutants
- 24.9. Transformation by Agrobacterium tumefaciens
- 25. Miscellaneous applications
- 25.1. In phytopathology
- 25.2. In plant breeding
- 25.3. The biosynthesis of substances in vitro
- 26. In vitro cloning of plants in the Netherlands
- 27. Index
- 28. Literature cited.