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190108s2019 xxu| s |||| 0|eng d |
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|a 9781493989881
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| 024 |
7 |
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|a 10.1007/978-1-4939-8988-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Calpain :
|b Methods and Protocols /
|c edited by Jeannette S. Messer.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Springer,
|c 2019.
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| 300 |
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|a XII, 276 p. 66 illus., 37 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1915
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| 505 |
0 |
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|a Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography -- Bacterial Expression and Purification of Calpains -- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity -- FRET-Based Assays to Determine Calpain Activity -- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer -- Determining Temporal and Spatial Expression of Calpains in Amphibians -- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis -- Expression and Activity of Calpain A in Drosophila melanogaster -- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1) -- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence -- CalCleaveMKL: A Tool for Calpain Cleavage Prediction -- Identification of Calpain-Activated protein Functions -- Production and Purification of Recombinant Calpastatin -- Isolation of Endogenous Calpastatin -- Immunoblotting for Calpastatin Expression -- Experimental Manipulation of Calpain Activity In Vitro -- In Vivo Calpain Knockdown using Delivery of siRNA -- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus -- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians -- Genetic Models of Calpain Deficiency and Ectopic Expression.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Messer, Jeannette S.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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