Calpain : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Springer,
2019.
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| Edition: | 1st ed. 2019. |
| Series: | Methods in Molecular Biology,
1915 |
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Table of Contents:
- Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography
- Bacterial Expression and Purification of Calpains
- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity
- FRET-Based Assays to Determine Calpain Activity
- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer
- Determining Temporal and Spatial Expression of Calpains in Amphibians
- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis
- Expression and Activity of Calpain A in Drosophila melanogaster
- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1)
- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
- CalCleaveMKL: A Tool for Calpain Cleavage Prediction
- Identification of Calpain-Activated protein Functions
- Production and Purification of Recombinant Calpastatin
- Isolation of Endogenous Calpastatin
- Immunoblotting for Calpastatin Expression
- Experimental Manipulation of Calpain Activity In Vitro
- In Vivo Calpain Knockdown using Delivery of siRNA
- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus
- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
- Genetic Models of Calpain Deficiency and Ectopic Expression.