Calpain : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Messer, Jeannette S. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Springer, 2019.
Edition:1st ed. 2019.
Series:Methods in Molecular Biology, 1915
Subjects:
Table of Contents:
  • Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography
  • Bacterial Expression and Purification of Calpains
  • Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity
  • FRET-Based Assays to Determine Calpain Activity
  • In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer
  • Determining Temporal and Spatial Expression of Calpains in Amphibians
  • Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis
  • Expression and Activity of Calpain A in Drosophila melanogaster
  • Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1)
  • LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
  • CalCleaveMKL: A Tool for Calpain Cleavage Prediction
  • Identification of Calpain-Activated protein Functions
  • Production and Purification of Recombinant Calpastatin
  • Isolation of Endogenous Calpastatin
  • Immunoblotting for Calpastatin Expression
  • Experimental Manipulation of Calpain Activity In Vitro
  • In Vivo Calpain Knockdown using Delivery of siRNA
  • In Vivo Expression of Mutant Calpains in the Eye using Lentivirus
  • Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
  • Genetic Models of Calpain Deficiency and Ectopic Expression.