Antibody Engineering : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 3rd ed. 2018. |
| Colección: | Methods in Molecular Biology,
1827 |
| Materias: |
Tabla de Contenidos:
- Antibody Design and Humanization via In Silico Modeling
- Antibody Affinity Maturation by Computational Design
- Use of IMGT® Databases and Tools for Antibody Engineering and Humanization
- Construction of Human Naïve Antibody Gene Libraries
- Construction of Synthetic Antibody Libraries
- Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments
- Display Technologies for Generation of Ig Single Variable Domains
- A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries
- Phage Display and Selections on Purified Antigens
- Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display
- Selection of Antibody Fragments against Structured DNA by Phage Display
- Selection of Antibody Fragments by Yeast Display
- Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display
- In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display
- Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood
- Expression of IgG Monoclonals with Engineered Immune Effector Functions
- An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines
- Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins
- Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases
- Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity
- Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0
- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
- Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development
- The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies
- Selection and Use of Intracellular Antibodies
- Site-Specific Radioactive Labeling of Nanobodies.