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181207s2019 xxu| s |||| 0|eng d |
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|a 9781493988617
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| 024 |
7 |
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|a 10.1007/978-1-4939-8861-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a BCL-2 Family Proteins :
|b Methods and Protocols /
|c edited by Evripidis Gavathiotis.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XI, 360 p. 96 illus., 66 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1877
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| 505 |
0 |
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|a Overview of BCL-2 Family Proteins and Therapeutic Potentials -- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins -- Application of Mito-Priming to Generate BCL-2 Addicted Cells -- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies -- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP) -- Investigating BCL-2 Family Protein Interactions in Yeast -- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family -- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP -- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family -- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol -- Quantification of BCL-2 Family Members by Flow Cytometry -- Methods to Probe Calcium Regulation by BCL-2 Family Members -- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK -- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage -- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs -- Cryo-Electron Microscopy to Study Bax Pores and MOMP -- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores -- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET -- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy -- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX).
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Gavathiotis, Evripidis.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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