BCL-2 Family Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1877 |
| Materias: |
Tabla de Contenidos:
- Overview of BCL-2 Family Proteins and Therapeutic Potentials
- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins
- Application of Mito-Priming to Generate BCL-2 Addicted Cells
- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies
- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)
- Investigating BCL-2 Family Protein Interactions in Yeast
- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family
- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP
- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family
- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol
- Quantification of BCL-2 Family Members by Flow Cytometry
- Methods to Probe Calcium Regulation by BCL-2 Family Members
- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK
- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage
- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs
- Cryo-Electron Microscopy to Study Bax Pores and MOMP
- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores
- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET
- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy
- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX).