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100301s2006 xxu| s |||| 0|eng d |
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|a 9781592599691
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|a 10.1385/1592599699
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Breast Cancer Research Protocols /
|c edited by Susan A. Brooks, Adrian Harris.
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250 |
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|a 1st ed. 2006.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2006.
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|a XIV, 518 p. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Medicine,
|v 120
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|a Preparation of Cell and Tissue Samples -- Breast Tissue Banking -- Frozen Biopsy Collection and Storage -- Serial Biopsies/Fine-Needle Aspirates and Their Assessment -- Breast Tissue Microarrays -- Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA -- Isolation of RNA From Tumor Samples -- Isolation of DNA From Tumor Samples -- Laser-Assisted Microdissection and Isolation of DNA and RNA -- Markers of Clinical Outcome and Prognosis -- Traditional and Established Indicators of Prognosis and Treatment Success -- Sentinel Lymph Node Biopsy in Breast Cancer -- Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer -- Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections -- Markers of Apoptosis -- Quantitative Angiogenesis in Breast Cancer -- Analysis of Tumor-Derived Proteins and Antigens -- Immunohistochemistry -- Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry -- SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research -- Breast Cancer Proteomics Using Two-Dimensional Electrophoresis -- Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA -- Analysis of Genes and Gene Expression in Tumor Specimens -- Fluorescence In Situ Hybridization and Comparative Genomic Hybridization -- Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues -- Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer -- In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression -- Quantitation of RNA by Ribonuclease Protection Assay -- Identification of Steroid Hormone-Regulated Genes in Breast Cancer -- Sequencing of the Tumor Suppressor Gene TP 53 -- Expression Profiling Using cDNA Microarrays -- Gene Expression Analysis Using Filter cDNA Microarrays -- Studying Cancer Cell Behavior in Vitro and In Vivo -- Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events -- Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage -- Models of Hormone Resistance In Vitro and In Vivo -- Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems -- In Vivo Xenograft Models of Breast Cancer Metastasis -- Neoadjuvant Endocrine Therapy Models -- Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis.
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|a Oncology .
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|a Oncology.
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1 |
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|a Brooks, Susan A.
|e editor.
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700 |
1 |
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|a Harris, Adrian.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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