Breast Cancer Research Protocols /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edición: | 1st ed. 2006. |
| Colección: | Methods in Molecular Medicine,
120 |
| Materias: |
Tabla de Contenidos:
- Preparation of Cell and Tissue Samples
- Breast Tissue Banking
- Frozen Biopsy Collection and Storage
- Serial Biopsies/Fine-Needle Aspirates and Their Assessment
- Breast Tissue Microarrays
- Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA
- Isolation of RNA From Tumor Samples
- Isolation of DNA From Tumor Samples
- Laser-Assisted Microdissection and Isolation of DNA and RNA
- Markers of Clinical Outcome and Prognosis
- Traditional and Established Indicators of Prognosis and Treatment Success
- Sentinel Lymph Node Biopsy in Breast Cancer
- Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer
- Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections
- Markers of Apoptosis
- Quantitative Angiogenesis in Breast Cancer
- Analysis of Tumor-Derived Proteins and Antigens
- Immunohistochemistry
- Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry
- SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research
- Breast Cancer Proteomics Using Two-Dimensional Electrophoresis
- Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA
- Analysis of Genes and Gene Expression in Tumor Specimens
- Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
- Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
- Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer
- In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression
- Quantitation of RNA by Ribonuclease Protection Assay
- Identification of Steroid Hormone-Regulated Genes in Breast Cancer
- Sequencing of the Tumor Suppressor Gene TP 53
- Expression Profiling Using cDNA Microarrays
- Gene Expression Analysis Using Filter cDNA Microarrays
- Studying Cancer Cell Behavior in Vitro and In Vivo
- Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events
- Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
- Models of Hormone Resistance In Vitro and In Vivo
- Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems
- In Vivo Xenograft Models of Breast Cancer Metastasis
- Neoadjuvant Endocrine Therapy Models
- Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis.