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190325s2019 xxu| s |||| 0|eng d |
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|a 9781493991709
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|a 10.1007/978-1-4939-9170-9
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a CRISPR Gene Editing :
|b Methods and Protocols /
|c edited by Yonglun Luo.
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|a 1st ed. 2019.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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300 |
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|a XI, 362 p. 76 illus., 61 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1961
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|a CRISPR-gRNA Design -- Tracking CRISPR’s Footprints -- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER -- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA -- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check -- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC) -- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery -- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes -- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9 -- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells -- Rapid and Efficient Gene Deletion by CRISPR/Cas9 -- Genome Editing in Mice -- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol -- Gene Editing in Primary Cells of Cattle and Pig -- Toward In Vivo Gene Therapy Using CRISPR -- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery -- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection -- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.
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|a Human genetics.
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|a Genetic engineering.
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|a Human Genetics.
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|a Genetic Engineering.
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|a Luo, Yonglun.
|e editor.
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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