CRISPR Gene Editing : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Luo, Yonglun. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1961
Materias:
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020 |a 9781493991709 
024 7 |a 10.1007/978-1-4939-9170-9  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a CRISPR Gene Editing :  |b Methods and Protocols /  |c edited by Yonglun Luo. 
250 |a 1st ed. 2019. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2019. 
300 |a XI, 362 p. 76 illus., 61 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1961 
505 0 |a CRISPR-gRNA Design -- Tracking CRISPR’s Footprints -- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER -- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA -- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check -- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC) -- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery -- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes -- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9 -- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells -- Rapid and Efficient Gene Deletion by CRISPR/Cas9 -- Genome Editing in Mice -- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol -- Gene Editing in Primary Cells of Cattle and Pig -- Toward In Vivo Gene Therapy Using CRISPR -- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery -- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection -- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes. 
650 0 |a Human genetics. 
650 0 |a Genetic engineering. 
650 1 4 |a Human Genetics. 
650 2 4 |a Genetic Engineering. 
700 1 |a Luo, Yonglun.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)