CRISPR Gene Editing : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1961 |
| Materias: |
Tabla de Contenidos:
- CRISPR-gRNA Design
- Tracking CRISPR’s Footprints
- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER
- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA
- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check
- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC)
- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery
- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors
- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs
- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes
- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9
- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells
- Rapid and Efficient Gene Deletion by CRISPR/Cas9
- Genome Editing in Mice
- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol
- Gene Editing in Primary Cells of Cattle and Pig
- Toward In Vivo Gene Therapy Using CRISPR
- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery
- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection
- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.