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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592598106
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| 024 |
7 |
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|a 10.1385/1592598102
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Cartilage and Osteoarthritis /
|c edited by Massimo Sabatini, Philippe Pastoureau, FrTdTric De Ceuninck.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XIV, 358 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 100
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| 505 |
0 |
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|a Culture and Phenotyping of Chondrocytes in Primary Culture -- Culture of Chondrocytes in Alginate Beads -- Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines -- Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function -- Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype -- Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR -- Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies -- RNA Extraction From Cartilage -- Gene Expression Analysis in Cartilage by In Situ Hybridization -- Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis -- High-Efficiency Nonviral Transfection of Primary Chondrocytes -- In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors -- Changes of Chondrocyte Metabolism In Vitro -- Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry -- A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes -- Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants -- Production of Antibodies Against Degradative Neoepitopes in Aggrecan -- Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures -- Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes -- Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes -- Mechanical Loading of Chondrocytes Embedded in 3D Constructs -- In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage.
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| 650 |
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0 |
|a Endocrinology .
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| 650 |
1 |
4 |
|a Endocrinology.
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| 700 |
1 |
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|a Sabatini, Massimo.
|e editor.
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| 700 |
1 |
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|a Pastoureau, Philippe.
|e editor.
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| 700 |
1 |
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|a De Ceuninck, FrTdTric.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|