Cartilage and Osteoarthritis /
Autor Corporativo: | |
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Otros Autores: | , , |
Formato: | eBook |
Lenguaje: | English |
Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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Edición: | 1st ed. 2004. |
Colección: | Methods in Molecular Medicine,
100 |
Materias: |
Tabla de Contenidos:
- Culture and Phenotyping of Chondrocytes in Primary Culture
- Culture of Chondrocytes in Alginate Beads
- Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
- Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function
- Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype
- Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR
- Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies
- RNA Extraction From Cartilage
- Gene Expression Analysis in Cartilage by In Situ Hybridization
- Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis
- High-Efficiency Nonviral Transfection of Primary Chondrocytes
- In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors
- Changes of Chondrocyte Metabolism In Vitro
- Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry
- A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
- Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
- Production of Antibodies Against Degradative Neoepitopes in Aggrecan
- Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures
- Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes
- Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes
- Mechanical Loading of Chondrocytes Embedded in 3D Constructs
- In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage.