Cartilage and Osteoarthritis /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Sabatini, Massimo. (Editor ), Pastoureau, Philippe. (Editor ), De Ceuninck, FrTdTric. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Medicine, 100
Materias:
Tabla de Contenidos:
  • Culture and Phenotyping of Chondrocytes in Primary Culture
  • Culture of Chondrocytes in Alginate Beads
  • Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
  • Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function
  • Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype
  • Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR
  • Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies
  • RNA Extraction From Cartilage
  • Gene Expression Analysis in Cartilage by In Situ Hybridization
  • Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis
  • High-Efficiency Nonviral Transfection of Primary Chondrocytes
  • In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors
  • Changes of Chondrocyte Metabolism In Vitro
  • Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry
  • A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
  • Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
  • Production of Antibodies Against Degradative Neoepitopes in Aggrecan
  • Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures
  • Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes
  • Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes
  • Mechanical Loading of Chondrocytes Embedded in 3D Constructs
  • In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage.