Cell-Penetrating Peptides : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Langel, Ülo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 683
Materias:
Tabla de Contenidos:
  • Classes and prediction of cell-penetrating peptides
  • Penetratin story : an overview
  • Testing membrane interactions of CPPs
  • Interactions of amphipathic CPPs with model membranes
  • NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems
  • Measurements of the intracellular stability of CPPs
  • Tools for predicting binding and insertion of CPPs into lipid bilayers
  • Studies of proteoglycan involvement in CPP-mediated delivery
  • Uptake Kinetics of cell-penetrating peptides
  • Thermodynamics of lipid interactions with cell-penetrating peptides
  • Calcium and membrane repair
  • Mapping of protein transduction pathways with fluorescent microscopy
  • Insight into cell-entry mechanisms of CPPs by electron microscopy
  • Toxicity methods for CPPs
  • Comparison of CPP uptake methods
  • Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery
  • Mimicry of protein function with cell-penetrating peptides
  • Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides
  • Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models
  • Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display
  • Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis
  • Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism
  • CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy
  • PTD-DRBD siRNA delivery
  • A non-covalent peptide-based strategy for siRNA delivery
  • Application of PepFect peptides for the delivery of splice correcting oligonucleotides
  • Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin
  • Enhanced cellular delivery of cell-penetrating peptide –peptide nucleic acid conjugates by photochemical internalization (PCI)
  • Identification of homing peptides using the in vivo phage display technology
  • Measuring the action of CPP-siRNA conjugates in the lung
  • Intracellular delivery of nanoparticles with CPPs
  • Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery
  • Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application
  • PAIR technology: exon-specific RNA binding protein isolation in live cells
  • Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates
  • Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug
  • Application of a fusiogenic peptide GALA for intracellular delivery
  • Therapeutic Applications of Cell-Penetrating Peptides
  • Nonclinical and clinical experiences with CPP-based self assembling peptide systems in topical drug development.