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141118s2015 xxu| s |||| 0|eng d |
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|a 9781493918621
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| 024 |
7 |
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|a 10.1007/978-1-4939-1862-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Chromosomal Mutagenesis /
|c edited by Shondra M. Pruett-Miller.
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| 250 |
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|a 2nd ed. 2015.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XIV, 333 p. 22 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1239
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| 505 |
0 |
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|a Genome Editing by Targeted Chromosomal Mutagenesis -- piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells -- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy -- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA -- Genome Engineering Using Adeno-Associated Virus (AAV) -- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization -- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform -- Ligation-Independent Cloning (LIC) Assembly of TALENs -- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications -- Genome Engineering Using CRISPR-Cas9 System -- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases -- Endogenous Gene Tagging with Fluorescent Proteins -- Silencing Long Non-Coding RNAs with Genome-Editing Tools -- Gene Editing Using ssODNs with Engineered Endonucleases -- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases -- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells -- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models -- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection -- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Genetic engineering.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Genetic Engineering.
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| 700 |
1 |
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|a Pruett-Miller, Shondra M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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