Chromosomal Mutagenesis /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 2nd ed. 2015. |
| Colección: | Methods in Molecular Biology,
1239 |
| Materias: |
Tabla de Contenidos:
- Genome Editing by Targeted Chromosomal Mutagenesis
- piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells
- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy
- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA
- Genome Engineering Using Adeno-Associated Virus (AAV)
- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization
- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform
- Ligation-Independent Cloning (LIC) Assembly of TALENs
- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications
- Genome Engineering Using CRISPR-Cas9 System
- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases
- Endogenous Gene Tagging with Fluorescent Proteins
- Silencing Long Non-Coding RNAs with Genome-Editing Tools
- Gene Editing Using ssODNs with Engineered Endonucleases
- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases
- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells
- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models
- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection
- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.