Cyclooxygenases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ayoub, Samir S. (Editor ), Flower, Roderick J. (Editor ), Seed, Michael. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 644
Materias:
Tabla de Contenidos:
  • In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns
  • In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I
  • Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites
  • Purification of Recombinant Human COX-1 and COX-2
  • Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2
  • Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System
  • Peroxidase Active Site Activity Assay
  • Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes
  • Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors
  • In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates
  • Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site
  • In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization
  • Extraction and Measurement of Prostanoids and Isoprostanes
  • Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II
  • Prostanoid Extraction and Measurement
  • Measurement of 8-Iso-Prostaglandin F2? in Biological Fluids as a Measure of Lipid Peroxidation
  • In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease
  • In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III
  • Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms
  • Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats
  • Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception.