Fluorescence Spectroscopy and Microscopy : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Engelborghs, Yves. (Editor ), Visser, Antonie J.W.G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1076
Materias:
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040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Fluorescence Spectroscopy and Microscopy :  |b Methods and Protocols /  |c edited by Yves Engelborghs, Antonie J.W.G. Visser. 
250 |a 1st ed. 2014. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2014. 
300 |a XXI, 816 p. 244 illus., 171 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1076 
505 0 |a Part I: Steady-State Fluorescence Spectroscopy (SSFS). How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra -- Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions -- Quantitative Fluorescence Spectral Analysis of Protein Denaturation -- High-Pressure Fluorescence Applications -- Part II: Time-Resolved Fluorescence Spectroscopy (TRFS). Frequency Domain Fluorometry: Theory and Application -- Polar Plot Representation of Time-Resolved Fluorescence -- Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics -- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes -- Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins -- Global Analysis of Time-Resolved Fluorescence Data -- Nanometrology -- Upconversion Spectrophotofluorometry -- Subpicosecond Kerr-Gate Spectrofluorometry -- Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay -- Part III: Fluorescent Probe Development (FPD). Biosynthetic Incorporation of Trp Analogs in Proteins -- Optimization of Fluorescent Proteins -- Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family -- Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP). Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope -- Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim). A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging -- Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM). Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors -- Global Analysis of FRET- FLIM Data in Live Plant Cells -- Time-Resolved Fluorescence Anisotropy Imaging -- Multi-Modal Fluorescence Imaging Spectroscopy -- Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS). Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells -- Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells -- Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS) -- PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching -- Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes -- Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane -- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS -- Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy -- Brightness Experiments -- Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy -- Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy -- Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS). Single-Molecule Fluorescence of Nucleic Acids -- Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light. 
650 0 |a Radiology. 
650 0 |a Microscopy. 
650 0 |a Spectroscopy. 
650 1 4 |a Imaging / Radiology. 
650 2 4 |a Biological Microscopy. 
650 2 4 |a Spectroscopy/Spectrometry. 
700 1 |a Engelborghs, Yves.  |e editor. 
700 1 |a Visser, Antonie J.W.G.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks