Fluorescence Spectroscopy and Microscopy : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1076 |
| Materias: |
Tabla de Contenidos:
- Part I: Steady-State Fluorescence Spectroscopy (SSFS). How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
- Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
- Quantitative Fluorescence Spectral Analysis of Protein Denaturation
- High-Pressure Fluorescence Applications
- Part II: Time-Resolved Fluorescence Spectroscopy (TRFS). Frequency Domain Fluorometry: Theory and Application
- Polar Plot Representation of Time-Resolved Fluorescence
- Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics
- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
- Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins
- Global Analysis of Time-Resolved Fluorescence Data
- Nanometrology
- Upconversion Spectrophotofluorometry
- Subpicosecond Kerr-Gate Spectrofluorometry
- Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay
- Part III: Fluorescent Probe Development (FPD). Biosynthetic Incorporation of Trp Analogs in Proteins
- Optimization of Fluorescent Proteins
- Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
- Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP). Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
- Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim). A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging
- Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM). Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors
- Global Analysis of FRET- FLIM Data in Live Plant Cells
- Time-Resolved Fluorescence Anisotropy Imaging
- Multi-Modal Fluorescence Imaging Spectroscopy
- Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS). Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells
- Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells
- Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)
- PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching
- Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes
- Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane
- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS
- Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy
- Brightness Experiments
- Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy
- Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy
- Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS). Single-Molecule Fluorescence of Nucleic Acids
- Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light.