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150616s2015 xxu| s |||| 0|eng d |
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|a 9781493927609
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| 024 |
7 |
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|a 10.1007/978-1-4939-2760-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Glyco-Engineering :
|b Methods and Protocols /
|c edited by Alexandra Castilho.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XVI, 439 p. 79 illus., 52 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1321
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| 505 |
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|a Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria -- Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli -- GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes -- Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli -- Glyco-Engineering O-Antigene-Based Vaccines and Diagnostics in E. coli -- Progress in Yeast Glycosylation Engineering -- Protein Production with a Pichia pastoris OCH1 Knock-Out Strain in Fed-Batch Mode -- Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology -- Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins -- An Overview and History of Glyco-Engineering in Insect Expression Systems -- SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation -- Engineering Sialic Acid Synthesis Ability in Insect Cells -- Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-N-Acetylglucosaminidase and Expression of β-1,4-Galactosyltransferase -- N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements -- Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors -- Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation -- Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-Antennary Sialylated Proteins -- Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation -- Assembly of Multigene Constructs Using Golden Gate Cloning -- Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells -- Glycan Remodelling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells -- Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants -- Metabolic Glyco-Engineering in Eukaryotic Cells and Selected Applications -- Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis -- Chemoenzymatic Glyco-Engineering of Monoclonal Antibodies -- Chemical Polysialylation of Recombinant Human Proteins -- Site Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) -- Mass Spectrometric Analysis of Oligo- and Polysialic Acids -- Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.
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| 650 |
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|a Proteins .
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| 650 |
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4 |
|a Protein Science.
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| 700 |
1 |
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|a Castilho, Alexandra.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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