Glyco-Engineering : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Castilho, Alexandra. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1321
Materias:
Tabla de Contenidos:
  • Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria
  • Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli
  • GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes
  • Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli
  • Glyco-Engineering O-Antigene-Based Vaccines and Diagnostics in E. coli
  • Progress in Yeast Glycosylation Engineering
  • Protein Production with a Pichia pastoris OCH1 Knock-Out Strain in Fed-Batch Mode
  • Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology
  • Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins
  • An Overview and History of Glyco-Engineering in Insect Expression Systems
  • SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation
  • Engineering Sialic Acid Synthesis Ability in Insect Cells
  • Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-N-Acetylglucosaminidase and Expression of β-1,4-Galactosyltransferase
  • N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements
  • Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors
  • Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
  • Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-Antennary Sialylated Proteins
  • Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation
  • Assembly of Multigene Constructs Using Golden Gate Cloning
  • Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells
  • Glycan Remodelling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells
  • Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants
  • Metabolic Glyco-Engineering in Eukaryotic Cells and Selected Applications
  • Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis
  • Chemoenzymatic Glyco-Engineering of Monoclonal Antibodies
  • Chemical Polysialylation of Recombinant Human Proteins
  • Site Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)
  • Mass Spectrometric Analysis of Oligo- and Polysialic Acids
  • Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.