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02767nam a22003015i 4500 |
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000280669 |
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20211116112742.0 |
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cr nn 008mamaa |
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100301s2003 xxu| s |||| 0|eng d |
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|a 9781592593019
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| 024 |
7 |
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|a 10.1385/1592593011
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a E. coli Gene Expression Protocols /
|c edited by Peter E. Vaillancourt.
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| 250 |
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|a 1st ed. 2003.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a XI, 347 p. 129 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 205
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| 505 |
0 |
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|a Cold-Inducible Promoters for Heterologous Protein Expression -- Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells -- A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications -- Purification of Recombinant Proteins from E. coli by Engineered Inteins -- Calmodulin as an Affinity Purification Tag -- Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification -- Maltose-Binding Protein as a Solubility Enhancer -- Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli -- Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli -- Assessment of Protein Folding/Solubility in Live Cells -- Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression -- High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins -- Co-Expression of Proteins in E. coli Using Dual Expression Vectors -- Small-Molecule Affinity-Based Matrices for Rapid Protein Purification -- Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli -- Screening Peptide/Protein Libraries Fused to the ? Repressor DNA-Binding Domain in E. coli Cells -- Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System -- Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions -- Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli -- Hyperphage -- Combinatorial Biosynthesis of Novel Carotenoids in E. coli -- Using Transcriptional-Based Systems for In Vivo Enzyme Screening -- Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis.
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| 650 |
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|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Vaillancourt, Peter E.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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