E. coli Gene Expression Protocols /

E. coli Gene Expression Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vaillancourt, Peter E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2003.
Edición:1st ed. 2003.
Colección:Methods in Molecular Biology, 205
Materias:
Biochemistry.
Cell biology.
Biochemistry, general.
Cell Biology.
Tabla de Contenidos:
  • Cold-Inducible Promoters for Heterologous Protein Expression
  • Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells
  • A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications
  • Purification of Recombinant Proteins from E. coli by Engineered Inteins
  • Calmodulin as an Affinity Purification Tag
  • Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification
  • Maltose-Binding Protein as a Solubility Enhancer
  • Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli
  • Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli
  • Assessment of Protein Folding/Solubility in Live Cells
  • Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression
  • High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins
  • Co-Expression of Proteins in E. coli Using Dual Expression Vectors
  • Small-Molecule Affinity-Based Matrices for Rapid Protein Purification
  • Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli
  • Screening Peptide/Protein Libraries Fused to the ? Repressor DNA-Binding Domain in E. coli Cells
  • Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System
  • Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions
  • Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli
  • Hyperphage
  • Combinatorial Biosynthesis of Novel Carotenoids in E. coli
  • Using Transcriptional-Based Systems for In Vivo Enzyme Screening
  • Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis.

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