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100301s2003 xxu| s |||| 0|eng d |
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|a 9781592593958
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| 024 |
7 |
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|a 10.1385/159259395X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Directed Evolution Library Creation :
|b Methods and Protocols /
|c edited by Frances H. Arnold, George Georgiou.
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| 250 |
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|a 1st ed. 2003.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a X, 226 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 231
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| 505 |
0 |
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|a Mutagenesis and Recombination Methods -- Generating Mutant Libraries Using Error-Prone PCR -- Preparing Libraries in Escherichia coli -- Preparing Libraries in Saccharomyces cerevisiae -- Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) -- Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis -- Production of Randomly Mutated Plasmid Libraries Using Mutator Strains -- Evolution of Microorganisms Using Mutator Plasmids -- Random Insertion and Deletion Mutagenesis -- Random Oligonucleotide Mutagenesis -- Saturation Mutagenesis -- DNA Shuffling -- Family Shuffling with Single-Stranded DNA -- In Vitro DNA Recombination by Random Priming -- Staggered Extension Process (StEP) In Vitro Recombination -- RACHITT -- The Creation of ITCHY Hybrid Protein Libraries -- Preparation of SCRATCHY Hybrid Protein Libraries -- Sequence Homology-Independent Protein Recombination (SHIPREC) -- Producing Chimeric Genes by CLERY -- Analysis of Library Diversity -- Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization -- Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays -- Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Arnold, Frances H.
|e editor.
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| 700 |
1 |
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|a Georgiou, George.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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