Directed Evolution Library Creation : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 1st ed. 2003. |
| Colección: | Methods in Molecular Biology,
231 |
| Materias: |
Tabla de Contenidos:
- Mutagenesis and Recombination Methods
- Generating Mutant Libraries Using Error-Prone PCR
- Preparing Libraries in Escherichia coli
- Preparing Libraries in Saccharomyces cerevisiae
- Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)
- Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis
- Production of Randomly Mutated Plasmid Libraries Using Mutator Strains
- Evolution of Microorganisms Using Mutator Plasmids
- Random Insertion and Deletion Mutagenesis
- Random Oligonucleotide Mutagenesis
- Saturation Mutagenesis
- DNA Shuffling
- Family Shuffling with Single-Stranded DNA
- In Vitro DNA Recombination by Random Priming
- Staggered Extension Process (StEP) In Vitro Recombination
- RACHITT
- The Creation of ITCHY Hybrid Protein Libraries
- Preparation of SCRATCHY Hybrid Protein Libraries
- Sequence Homology-Independent Protein Recombination (SHIPREC)
- Producing Chimeric Genes by CLERY
- Analysis of Library Diversity
- Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization
- Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays
- Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays.