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161206s2017 xxu| s |||| 0|eng d |
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|a 9781493964246
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| 024 |
7 |
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|a 10.1007/978-1-4939-6424-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a ERK Signaling :
|b Methods and Protocols /
|c edited by Gerardo Jimenez.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XII, 440 p. 40 illus., 21 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1487
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| 505 |
0 |
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|a How Genetics has Helped Piece Together the MAPK Signaling Pathway -- In Vitro Enzyme Kinetics Analysis of EGFR -- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells -- Structural Studies of ERK2 Protein Complexes -- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk -- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues -- Detection and Functional Analysis of SUMO-Modified MEK -- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag -- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates -- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE -- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation -- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions -- The Nuclear Translocation of ERK -- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) -- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors -- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers -- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells -- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling -- 3D Organotypic Culture Model to Study Components of ERK Signaling -- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway -- Genome-Wide Analysis of RAS/ERK Signaling Targets -- Probing Chromatin Modifications in Response to ERK Signaling -- Analyzing pERK Activation during Planarian Regeneration -- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development -- Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images -- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila -- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis -- Modeling RASopathies with Genetically Modified Mouse Models -- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Jimenez, Gerardo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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