ERK Signaling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1487 |
| Materias: |
Tabla de Contenidos:
- How Genetics has Helped Piece Together the MAPK Signaling Pathway
- In Vitro Enzyme Kinetics Analysis of EGFR
- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells
- Structural Studies of ERK2 Protein Complexes
- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
- Detection and Functional Analysis of SUMO-Modified MEK
- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
- The Nuclear Translocation of ERK
- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
- 3D Organotypic Culture Model to Study Components of ERK Signaling
- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
- Genome-Wide Analysis of RAS/ERK Signaling Targets
- Probing Chromatin Modifications in Response to ERK Signaling
- Analyzing pERK Activation during Planarian Regeneration
- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
- Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila
- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis
- Modeling RASopathies with Genetically Modified Mouse Models
- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.