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121116s2013 xxu| s |||| 0|eng d |
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|a 9781627031769
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|a 10.1007/978-1-62703-176-9
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Electron Crystallography of Soluble and Membrane Proteins :
|b Methods and Protocols /
|c edited by Ingeborg Schmidt-Krey, Yifan Cheng.
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|a 1st ed. 2013.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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|a XIII, 586 p. 151 illus., 63 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 955
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|a Introduction to Electron Crystallography -- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis -- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis -- Monolayer Two-Dimensional Crystallization -- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples -- Low Dose Techniques and Cryo-Electron Microscopy -- Grid Preparation for Cryo-Electron Microscopy -- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins -- The Collection of High-Resolution Electron Diffraction Data -- Image Processing of 2D Crystal Images -- Merging of Image Data in Electron Crystallography -- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals -- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy -- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement -- High-Throughput Methods for Electron Crystallography -- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening -- Automation of Data Acquisition in Electron Crystallography -- Automation of Image Processing in Electron Crystallography -- Choice and Maintenance of Equipment for Electron Crystallography -- Future Developments in Instrumentation for Electron Crystallography -- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction -- Single Particle Electron Microscopy -- Electron Tomography of Paracrystalline 2D Arrays -- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy -- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography -- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy -- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography -- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples -- Processing of Electron Diffraction Patterns with the XDP Program -- Future Directions of Electron Crystallography.
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|a Proteins .
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|a Microscopy.
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|a Protein Science.
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|a Biological Microscopy.
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|a Schmidt-Krey, Ingeborg.
|e editor.
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|a Cheng, Yifan.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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