Electron Crystallography of Soluble and Membrane Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Schmidt-Krey, Ingeborg. (Editor ), Cheng, Yifan. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 955
Materias:
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024 7 |a 10.1007/978-1-62703-176-9  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Electron Crystallography of Soluble and Membrane Proteins :  |b Methods and Protocols /  |c edited by Ingeborg Schmidt-Krey, Yifan Cheng. 
250 |a 1st ed. 2013. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2013. 
300 |a XIII, 586 p. 151 illus., 63 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 955 
505 0 |a Introduction to Electron Crystallography -- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis -- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis -- Monolayer Two-Dimensional Crystallization -- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples -- Low Dose Techniques and Cryo-Electron Microscopy -- Grid Preparation for Cryo-Electron Microscopy -- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins -- The Collection of High-Resolution Electron Diffraction Data -- Image Processing of 2D Crystal Images -- Merging of Image Data in Electron Crystallography -- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals -- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy -- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement -- High-Throughput Methods for Electron Crystallography -- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening -- Automation of Data Acquisition in Electron Crystallography -- Automation of Image Processing in Electron Crystallography -- Choice and Maintenance of Equipment for Electron Crystallography -- Future Developments in Instrumentation for Electron Crystallography -- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction -- Single Particle Electron Microscopy -- Electron Tomography of Paracrystalline 2D Arrays -- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy -- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography -- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy -- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography -- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples -- Processing of Electron Diffraction Patterns with the XDP Program -- Future Directions of Electron Crystallography. 
650 0 |a Proteins . 
650 0 |a Microscopy. 
650 1 4 |a Protein Science. 
650 2 4 |a Biological Microscopy. 
700 1 |a Schmidt-Krey, Ingeborg.  |e editor. 
700 1 |a Cheng, Yifan.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks