Electron Crystallography of Soluble and Membrane Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
955 |
| Materias: |
Tabla de Contenidos:
- Introduction to Electron Crystallography
- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis
- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis
- Monolayer Two-Dimensional Crystallization
- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples
- Low Dose Techniques and Cryo-Electron Microscopy
- Grid Preparation for Cryo-Electron Microscopy
- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins
- The Collection of High-Resolution Electron Diffraction Data
- Image Processing of 2D Crystal Images
- Merging of Image Data in Electron Crystallography
- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals
- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy
- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement
- High-Throughput Methods for Electron Crystallography
- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening
- Automation of Data Acquisition in Electron Crystallography
- Automation of Image Processing in Electron Crystallography
- Choice and Maintenance of Equipment for Electron Crystallography
- Future Developments in Instrumentation for Electron Crystallography
- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction
- Single Particle Electron Microscopy
- Electron Tomography of Paracrystalline 2D Arrays
- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy
- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography
- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy
- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography
- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples
- Processing of Electron Diffraction Patterns with the XDP Program
- Future Directions of Electron Crystallography.