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100301s2002 xxu| s |||| 0|eng d |
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|a 9781592592814
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| 024 |
7 |
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|a 10.1385/1592592813
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a GTPase Protocols :
|b The Ras Superfamily /
|c edited by Ed Manser, Thomas Leung.
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| 250 |
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|a 1st ed. 2002.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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| 300 |
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|a XI, 267 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 189
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| 505 |
0 |
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|a General Protocols -- The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases -- Preparation of GTPases for Structural and Biophysical Analysis -- Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes -- Fluorescence Methods in the Study of Small GTP-Binding Proteins -- Specific Methods -- Ras and Rac as Activators of Reactive Oxygen Species (ROS) -- Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs -- A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays -- Determination of the Activity of Rho-Like GTPases in Cells -- of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases -- Effects of Rho Family GTPases on Cell-Cell Adhesion -- Cell Motility and Invasion Assays -- Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases -- Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase -- Preparation of Myristoylated Arf1 and Arf6 Proteins -- ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins -- Arf6 and its Role in Cytoskeletal Modulation -- ARF GTPase-Activating Protein 1 -- The Use of Permeabilized Cell Systems to Study Nuclear Transport -- Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes -- Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Manser, Ed.
|e editor.
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| 700 |
1 |
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|a Leung, Thomas.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|