GTPase Protocols : The Ras Superfamily /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
|
| Edición: | 1st ed. 2002. |
| Colección: | Methods in Molecular Biology,
189 |
| Materias: |
Tabla de Contenidos:
- General Protocols
- The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases
- Preparation of GTPases for Structural and Biophysical Analysis
- Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes
- Fluorescence Methods in the Study of Small GTP-Binding Proteins
- Specific Methods
- Ras and Rac as Activators of Reactive Oxygen Species (ROS)
- Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
- A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
- Determination of the Activity of Rho-Like GTPases in Cells
- of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases
- Effects of Rho Family GTPases on Cell-Cell Adhesion
- Cell Motility and Invasion Assays
- Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
- Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase
- Preparation of Myristoylated Arf1 and Arf6 Proteins
- ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
- Arf6 and its Role in Cytoskeletal Modulation
- ARF GTPase-Activating Protein 1
- The Use of Permeabilized Cell Systems to Study Nuclear Transport
- Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes
- Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton.