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100301s2003 xxu| s |||| 0|eng d |
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|a 9781592594092
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| 024 |
7 |
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|a 10.1385/1592594093
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a E. coli Plasmid Vectors :
|b Methods and Applications /
|c edited by Nicola Casali, Andrew Preston.
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| 250 |
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|a 1st ed. 2003.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a XII, 316 p. :
|b online resource.
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| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 235
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| 505 |
0 |
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|a The Function and Organization of Plasmids -- Choosing a Cloning Vector -- Escherichia coli Host Strains -- Chemical Transformation of E. coli -- Electroporation of E. coli -- DNA Transfer by Bacterial Conjugation -- Cosmid Packaging and Infection of E. coli -- Isolation of Plasmids from E. coli by Alkaline Lysis -- Isolation of Plasmids from E. coli by Boiling Lysis -- High-Purity Plasmid Isolation Using Silica Oxide -- High-Throughput Plasmid Extraction Using Microtiter Plates -- Isolation of Cosmid and BAC DNA from E. coli -- Preparation of Single-Stranded DNA from Phagemid Vectors -- Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects -- Cloning in Plasmid Vectors -- Extraction of DNA from Agarose Gels -- Cloning PCR Products with T-Vectors -- Construction of Genomic Libraries in ?-Vectors -- Rapid Screening of Recombinant Plasmids -- Restriction Analysis of Recombinant Plasmids -- Screening Recombinant DNA Libraries -- Sequencing Using Fluorescent-Labeled Nucleotides -- Site-Directed Mutagenesis Using the Megaprimer Method -- Site-Directed Mutagenesis by Inverse PCR -- Creating Nested DNA Deletions Using Exonuclease III -- Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs -- In Vitro Transcription and Translation -- Vectors for the Expression of Recombinant Proteins in E. coli -- Expression of Recombinant Proteins From lac Promoters -- Plasmid-Based Reporter Genes -- Plasmid-Based Reporter Genes.
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| 650 |
|
0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Casali, Nicola.
|e editor.
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| 700 |
1 |
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|a Preston, Andrew.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
|