E. coli Plasmid Vectors : Methods and Applications /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 1st ed. 2003. |
| Colección: | Methods in Molecular Biology,
235 |
| Materias: |
Tabla de Contenidos:
- The Function and Organization of Plasmids
- Choosing a Cloning Vector
- Escherichia coli Host Strains
- Chemical Transformation of E. coli
- Electroporation of E. coli
- DNA Transfer by Bacterial Conjugation
- Cosmid Packaging and Infection of E. coli
- Isolation of Plasmids from E. coli by Alkaline Lysis
- Isolation of Plasmids from E. coli by Boiling Lysis
- High-Purity Plasmid Isolation Using Silica Oxide
- High-Throughput Plasmid Extraction Using Microtiter Plates
- Isolation of Cosmid and BAC DNA from E. coli
- Preparation of Single-Stranded DNA from Phagemid Vectors
- Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects
- Cloning in Plasmid Vectors
- Extraction of DNA from Agarose Gels
- Cloning PCR Products with T-Vectors
- Construction of Genomic Libraries in ?-Vectors
- Rapid Screening of Recombinant Plasmids
- Restriction Analysis of Recombinant Plasmids
- Screening Recombinant DNA Libraries
- Sequencing Using Fluorescent-Labeled Nucleotides
- Site-Directed Mutagenesis Using the Megaprimer Method
- Site-Directed Mutagenesis by Inverse PCR
- Creating Nested DNA Deletions Using Exonuclease III
- Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs
- In Vitro Transcription and Translation
- Vectors for the Expression of Recombinant Proteins in E. coli
- Expression of Recombinant Proteins From lac Promoters
- Plasmid-Based Reporter Genes
- Plasmid-Based Reporter Genes.