Cytochrome P450 Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Phillips, Ian R. (Editor ), Shephard, Elizabeth A. (Editor ), Ortiz de Montellano, Paul R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:3rd ed. 2013.
Colección:Methods in Molecular Biology,
Materias:
Tabla de Contenidos:
  • Bioluminescent Assays for Cytochrome P450 Enzymes. In Vitro Drug-Drug Interaction Assay
  • High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening
  • The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
  • High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators
  • Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches
  • Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis
  • An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins
  • Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
  • Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes
  • Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles
  • Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells
  • Screening for Cytochrome P450 Reactivity with a Reporter Enzyme
  • High-Throughput Fluorescence Assay of Cytochrome P450 3A4
  • Targeted Protein Capture for Analysis of Electrophile-Protein Adducts
  • DNA Shuffling of Cytochrome P450 Enzymes
  • Measurement of P450 Difference Spectra Using Intact Cells
  • DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production
  • P450 oxidoreductase – Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein
  • LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450
  • Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database
  • Simultaneous in vivo Phenotyping of CYP Enzymes
  • Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting
  • Isolation of Mouse Hepatocytes
  • Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies.