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100301s2003 xxu| s |||| 0|eng d |
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|a 9781592593965
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| 024 |
7 |
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|a 10.1385/1592593968
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Directed Enzyme Evolution :
|b Screening and Selection Methods /
|c edited by Frances H. Arnold, George Georgiou.
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| 250 |
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|a 1st ed. 2003.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a XV, 383 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 230
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| 505 |
0 |
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|a Genetic Selections -- Genetic Complementation Protocols -- Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase -- Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast -- Autogene Selections -- Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase -- Proside -- Minimization of Proteins by Random Fragmentation and Selection -- Screens for Enzymes -- Evaluating a Screen and Analysis of Mutant Libraries -- Screening Mutant Libraries in Saccharomyces cerevisiae -- Solid-Phase Screening Using Digital Image Analysis -- Screening for Thermostability -- High-Throughput Screening of Mutant ?-Amylase Libraries for Increased Activity at 129°C -- High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450 -- High-Throughput Screen for Aromatic Hydroxylation -- Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes -- High-Throughput Screens Based on NAD(P)H Depletion -- High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases -- Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase -- Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation -- Colorimetric Assays for Screening Laccases -- pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity -- A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase -- Detection of Aromatic ?-Hydroxyketones with Tetrazolium Salts -- Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination -- Screening and Selection Strategies for Disulfide Isomerase Activity -- An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations -- Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes -- Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS) -- Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities -- High-Throughput FACS Method for Directed Evolution of Substrate Specificity -- Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Arnold, Frances H.
|e editor.
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| 700 |
1 |
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|a Georgiou, George.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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