Directed Enzyme Evolution : Screening and Selection Methods /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 1st ed. 2003. |
| Colección: | Methods in Molecular Biology,
230 |
| Materias: |
Tabla de Contenidos:
- Genetic Selections
- Genetic Complementation Protocols
- Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
- Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
- Autogene Selections
- Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase
- Proside
- Minimization of Proteins by Random Fragmentation and Selection
- Screens for Enzymes
- Evaluating a Screen and Analysis of Mutant Libraries
- Screening Mutant Libraries in Saccharomyces cerevisiae
- Solid-Phase Screening Using Digital Image Analysis
- Screening for Thermostability
- High-Throughput Screening of Mutant ?-Amylase Libraries for Increased Activity at 129°C
- High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
- High-Throughput Screen for Aromatic Hydroxylation
- Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes
- High-Throughput Screens Based on NAD(P)H Depletion
- High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases
- Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
- Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation
- Colorimetric Assays for Screening Laccases
- pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity
- A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
- Detection of Aromatic ?-Hydroxyketones with Tetrazolium Salts
- Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination
- Screening and Selection Strategies for Disulfide Isomerase Activity
- An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations
- Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes
- Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS)
- Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities
- High-Throughput FACS Method for Directed Evolution of Substrate Specificity
- Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter.