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100301s2002 xxu| s |||| 0|eng d |
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|a 9781592591879
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024 |
7 |
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|a 10.1385/1592591876
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Cystic Fibrosis Methods and Protocols /
|c edited by William R. Skach.
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250 |
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|a 1st ed. 2002.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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300 |
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|a XVI, 615 p. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Medicine,
|v 70
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|a Genetics of Cystic Fibrosis -- CFTR Mutation Detection by Multiplex Heteroduplex (mHET) Analysis on MDE Gel -- cDNA Microarrays for Pharmacogenomic Analysis of Cystic Fibrosis -- Natural Animal Models of Human Genetic Diseases -- CFTR structure and Function: -- Electrophysiological Approach to Studying CFTR -- Quantitative Analysis of ATP-Dependent Gating of CFTR -- CFTR Regulation by Phosphorylation -- Transepithelial Measurements of Bicarbonate Secretion in Calu-3 Cells -- Transepithelial Impedance Analysis of Chloride Secretion -- Studies of the Molecular Basis for Cystic Fibrosis Using Purified Reconstituted CFTR Protein -- Probing CFTR Channel Structure and Function Using the Substituted-Cysteine-Accessibility Method -- Methods for the Study of Intermolecular and Intramolecular Interactions Regulating CFTR Function -- Fluorescent Indicator Methods to Assay Functional CFTR Expression in Cells -- Immunolocalization of CFTR in Intact Tissue and Cultured Cells -- Analysis of CFTR Trafficking and Polarization Using Green Fluorescent Protein and Confocal Microscopy -- CFTR Folding and Maturation in Cells -- Isolation of CFTR -- CFTR Expression and ER-Associated Degradation in Yeast -- Manipulating the Folding Pathway of ?F508 CFTR Using Chemical Chaperones -- CFTR Degradation and Aggregation -- In Vitro Reconstitution of CFTR Biogenesis and Degradation -- In Vitro CFTR Folding Assays -- Analysis of CFTR Endocytosis by Cell Surface Biotinylation -- CFTR Regulation of ENaC -- Yeast Two-Hybrid Identification and Analysis of Protein Interactions with CFTR -- Biochemical Assays for Studying Indirect Interactions Between CFTR and the Cytoskeleton -- CFTR-Associated ATP Transport and Release -- Pathophysiology of Cystic Fibrosis -- Inflammatory Mediators in CF Patients -- Bacterial Colonization and Infection in the CF Lung -- Antimicrobial Peptides and Proteins in the CF Airway -- Bacterial-Epithelial Interactions -- Thin-Film Measurements of Airway Surface Liquid Volume/Composition and Mucus Transport Rates In Vitro -- Murine Models of CF Airway Infection and Inflammation -- Analysis of Lipid Abnormalities in CF Mice -- Bioelectric Measurement of CFTR Function in Mice -- Xenograft Model of the CF Airway -- Development of Conditionally Immortalized Epithelial Cell Lines from CF and Non-CF Mice -- Technical Approaches to Analyze the In Vivo Ion Composition of Airway Surface Liquid -- Novel Therapeutic Approaches for Cystic Fibrosis -- Design of Gene Therapy Clinical Trials in CF Patients -- Formulation of Synthetic Vectors for Cystic Fibrosis Gene Therapy -- Adeno-Associated Viral Vectors for CF Gene Therapy.
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|a Respiratory organs—Diseases.
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650 |
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4 |
|a Pneumology/Respiratory System.
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700 |
1 |
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|a Skach, William R.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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