Cystic Fibrosis Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edición: | 1st ed. 2002. |
| Colección: | Methods in Molecular Medicine,
70 |
| Materias: |
Tabla de Contenidos:
- Genetics of Cystic Fibrosis
- CFTR Mutation Detection by Multiplex Heteroduplex (mHET) Analysis on MDE Gel
- cDNA Microarrays for Pharmacogenomic Analysis of Cystic Fibrosis
- Natural Animal Models of Human Genetic Diseases
- CFTR structure and Function:
- Electrophysiological Approach to Studying CFTR
- Quantitative Analysis of ATP-Dependent Gating of CFTR
- CFTR Regulation by Phosphorylation
- Transepithelial Measurements of Bicarbonate Secretion in Calu-3 Cells
- Transepithelial Impedance Analysis of Chloride Secretion
- Studies of the Molecular Basis for Cystic Fibrosis Using Purified Reconstituted CFTR Protein
- Probing CFTR Channel Structure and Function Using the Substituted-Cysteine-Accessibility Method
- Methods for the Study of Intermolecular and Intramolecular Interactions Regulating CFTR Function
- Fluorescent Indicator Methods to Assay Functional CFTR Expression in Cells
- Immunolocalization of CFTR in Intact Tissue and Cultured Cells
- Analysis of CFTR Trafficking and Polarization Using Green Fluorescent Protein and Confocal Microscopy
- CFTR Folding and Maturation in Cells
- Isolation of CFTR
- CFTR Expression and ER-Associated Degradation in Yeast
- Manipulating the Folding Pathway of ?F508 CFTR Using Chemical Chaperones
- CFTR Degradation and Aggregation
- In Vitro Reconstitution of CFTR Biogenesis and Degradation
- In Vitro CFTR Folding Assays
- Analysis of CFTR Endocytosis by Cell Surface Biotinylation
- CFTR Regulation of ENaC
- Yeast Two-Hybrid Identification and Analysis of Protein Interactions with CFTR
- Biochemical Assays for Studying Indirect Interactions Between CFTR and the Cytoskeleton
- CFTR-Associated ATP Transport and Release
- Pathophysiology of Cystic Fibrosis
- Inflammatory Mediators in CF Patients
- Bacterial Colonization and Infection in the CF Lung
- Antimicrobial Peptides and Proteins in the CF Airway
- Bacterial-Epithelial Interactions
- Thin-Film Measurements of Airway Surface Liquid Volume/Composition and Mucus Transport Rates In Vitro
- Murine Models of CF Airway Infection and Inflammation
- Analysis of Lipid Abnormalities in CF Mice
- Bioelectric Measurement of CFTR Function in Mice
- Xenograft Model of the CF Airway
- Development of Conditionally Immortalized Epithelial Cell Lines from CF and Non-CF Mice
- Technical Approaches to Analyze the In Vivo Ion Composition of Airway Surface Liquid
- Novel Therapeutic Approaches for Cystic Fibrosis
- Design of Gene Therapy Clinical Trials in CF Patients
- Formulation of Synthetic Vectors for Cystic Fibrosis Gene Therapy
- Adeno-Associated Viral Vectors for CF Gene Therapy.