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03262nam a22003015i 4500 |
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180309s2018 xxu| s |||| 0|eng d |
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|a 9781493977741
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| 024 |
7 |
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|a 10.1007/978-1-4939-7774-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Epigenome Editing :
|b Methods and Protocols /
|c edited by Albert Jeltsch, Marianne G. Rots.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XIV, 482 p. 83 illus., 69 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1767
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| 505 |
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|a Editing the Epigenome: Overview, Open Questions, and Directions of Future Development -- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing -- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities -- Generation of TALE-Based Designer Epigenome Modifiers -- Neuroepigenetic Editing -- Allele-Specific Epigenome Editing -- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox -- CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control -- Delivery of Designer Epigenome Modifiers into Primary Human T Cells -- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques -- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration -- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain -- Non-Viral Methodology for Efficient Co-Transfection -- Chromatin Immunoprecipitation in Human and Yeast Cells -- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis -- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis -- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data -- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution -- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing -- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS) -- Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching -- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing -- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions -- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells -- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Jeltsch, Albert.
|e editor.
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| 700 |
1 |
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|a Rots, Marianne G.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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