Epigenome Editing : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1767 |
| Materias: |
Tabla de Contenidos:
- Editing the Epigenome: Overview, Open Questions, and Directions of Future Development
- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing
- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
- Generation of TALE-Based Designer Epigenome Modifiers
- Neuroepigenetic Editing
- Allele-Specific Epigenome Editing
- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox
- CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control
- Delivery of Designer Epigenome Modifiers into Primary Human T Cells
- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques
- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration
- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain
- Non-Viral Methodology for Efficient Co-Transfection
- Chromatin Immunoprecipitation in Human and Yeast Cells
- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis
- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis
- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data
- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution
- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing
- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS)
- Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching
- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing
- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions
- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells
- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.